{"id":95764,"date":"2016-08-16T09:29:14","date_gmt":"2016-08-16T07:29:14","guid":{"rendered":"https:\/\/clinical.r-biopharm.com\/tecnologie\/real-time-pcr\/"},"modified":"2019-07-08T15:45:51","modified_gmt":"2019-07-08T13:45:51","slug":"real-time-pcr","status":"publish","type":"page","link":"https:\/\/devclinical.r-biopharm.com\/it\/tecnologie\/real-time-pcr\/","title":{"rendered":"Real-time PCR"},"content":{"rendered":"
[mk_page_section padding_top=”30″ padding_bottom=”30″ sidebar=”sidebar-1″][vc_column width=”2\/3″][vc_column_text]I metodi di diagnostica molecolare hanno rivoluzionato la diagnostica medica e sono diventati una componente essenziale dell\u2019odierna attivit\u00e0 dei laboratori di analisi.<\/strong><\/p>\n

In molte zone colpite da malattie infettive, i classici metodi di analisi come le colture e i dosaggi immunologici sono stati sostituiti o supportati dal metodo real-time PCR (Reazione a Catena della Polimerasi in tempo reale).<\/p>\n

Il principio della real-time PCR<\/h3>\n

La real-time PCR identifica in maniera diretta il DNA o l\u2019RNA di un agente patogeno e consente dunque una diagnosi precoce e altamente specifica, che permette di adottare rapidamente la terapia pi\u00f9 adeguata per il paziente individuale. Dopo aver isolato il DNA, i frammenti di gene di nostro interesse vengono amplificati (ampliconi) e, se presenti, identificati da una sonda fluorescente che opera per un processo di idrolisi. Il segnale di fluorescenza aumenta in proporzione alla quantit\u00e0 degli ampliconi generati, e viene registrato e quantificato dall\u2019unit\u00e0 ottica di uno strumento per real-time PCR.<\/p>\n

RIDA\u00aeGENE in breve<\/h3>\n

I kit RIDA\u00aeGENE di R-Biopharm sfruttano la tecnologia della real-time PCR per la diagnosi diretta e qualitativa di infezioni gastrointestinali, ospedaliere, respiratorie e sessualmente trasmesse (IST) e di altri parametri.[\/vc_column_text][mk_custom_list style=”mk-moon-circle-small”]\n

    \n
  • Sensibili e specifici: real-time PCR con tecnologia Taqman delle sonde a idrolisi<\/li>\n
  • Completi: tutti le componenti necessarie in un unico kit<\/li>\n
  • Affidabili: \u00e8 incluso un controllo di estrazione che verifica l\u2019eventuale inibizione del processo di PCR, monitora l\u2019integrit\u00e0 dei reagenti e conferma l\u2019efficienza della procedura di estrazione dell\u2019acido nucleico<\/li>\n
  • Flessibili: compatibili con i vari strumenti di real-time PCR, come LightCycler\u00ae, Rotor-Gene Q, ABI7500, Mx3005P e CFX96\u2122<\/li>\n
  • Efficienti: un unico profilo PCR per i saggi a RNA o a DNA<\/li>\n
  • Validati: marchiati CE-IVD, QCMD, INSTAND e facenti parte del processo di valutazione qualitativa UK NEQAS<\/li>\n<\/ul>\n[\/mk_custom_list][vc_cta h2=”Panoramica sui prodotti” shape=”square” style=”flat” color=”grey” add_button=”right” btn_title=”Scarica” btn_style=”flat” btn_shape=”square” btn_color=”inverse” btn_link=”url:https%3A%2F%2Fdevclinical.r-biopharm.com%2Fwp-content%2Fuploads%2Fsites%2F3%2F2018%2F06%2F2018-03_rida-gene-produktbersicht_4p_en_lores.pdf|target:%20_blank|”]Per maggiori informazioni sui nostri kit RIDA\u00aeGENE real-time PCR, consulta il nostro opuscolo gratuito.<\/p>\n[\/vc_cta][\/vc_column][vc_column width=”1\/3″][vc_column_text margin_bottom=”20″]\n

    Scegli un\u2019indicazione<\/h3>\n
    <\/div>[\/vc_column_text][vc_column_text margin_bottom=”20″]\n

    Scegli un settore diagnostico<\/h3>\n
    <\/div>[\/vc_column_text][vc_column_text margin_bottom=”40″]\n

    Scegli il tuo prodotto<\/h3>\n
      <\/ul><\/div>[\/vc_column_text][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#f7f7f7″ padding_top=”0″ padding_bottom=”0″ sidebar=”sidebar-1″][vc_column css=”.vc_custom_1469015765963{margin-top: 25px !important;padding-bottom: 5px !important;}”][vc_column_text margin_bottom=”20″]\n

      Real-time PCR – ultime notizie<\/h2>\n[\/vc_column_text][mk_blog_carousel cat=”233″][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#ffffff” padding_top=”0″ sidebar=”sidebar-1″][vc_column css=”.vc_custom_1467042052405{margin-top: 40px !important;}”][vc_column_text margin_bottom=”20″]\n

      Potrebbe interessarti anche<\/h2>\n[\/vc_column_text][vc_row_inner css=”.vc_custom_1468404266697{margin-bottom: 40px !important;}”][vc_column_inner width=”1\/3″ css=”.vc_custom_1468416557833{padding-bottom: 10px !important;padding-left: 0px !important;}”][mk_flipbox_rb taxonomy=”technology” tag_technology=”qpcr-de” min_height=”200″ button_bg_color=”#4d4d4d” button_bg_hover_color=”#ffffff” button_text=”More”][\/vc_column_inner][vc_column_inner width=”1\/3″ css=”.vc_custom_1468416552613{padding-right: 7.5px !important;padding-bottom: 10px !important;padding-left: 7.5px !important;}”][mk_flipbox_rb taxonomy=”technology” tag_technology=”qpcr-de” offset=”1″ min_height=”200″ button_bg_color=”#4d4d4d” button_bg_hover_color=”#ffffff” button_text=”More”][\/vc_column_inner][vc_column_inner width=”1\/3″ css=”.vc_custom_1468416547451{padding-right: 0px !important;padding-bottom: 10px !important;}”][mk_flipbox_rb taxonomy=”technology” tag_technology=”qpcr-de” offset=”2″ min_height=”200″ button_bg_color=”#4d4d4d” button_bg_hover_color=”#ffffff” button_text=”More”][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#f7f7f7″ padding_top=”20″ padding_bottom=”20″ sidebar=”sidebar-1″][vc_column css=”.vc_custom_1468415774645{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column_text disable_pattern=”false” align=”center”]\n

      Real-time PCR – supporto<\/h2>\n

      Hai domande? Le nostre competenze sono a tua disposizione! La nostra assistenza \u00e8 garanzia di successo per i test diagnostici della tua azienda[\/vc_column_text][mk_button dimension=”flat” url=”\/it\/contatti\/” align=”center” margin_bottom=”” bg_color=”#4d4d4d” btn_hover_bg=”#ffffff” btn_hover_txt_color=”#4d4d4d”]Contattaci[\/mk_button][\/vc_column][\/mk_page_section]\n<\/div>","protected":false},"excerpt":{"rendered":"

      [mk_page_section padding_top=”30″ padding_bottom=”30″ sidebar=”sidebar-1″][vc_column width=”2\/3″][vc_column_text]I metodi di diagnostica molecolare hanno rivoluzionato la diagnostica medica e sono diventati una componente essenziale dell\u2019odierna attivit\u00e0 dei laboratori di analisi. In molte zone colpite da malattie infettive, i classici metodi di analisi come le colture e i dosaggi immunologici sono stati sostituiti o supportati dal metodo real-time PCR (Reazione […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":95629,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":"","_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","footnotes":""},"acf":[],"yoast_head":"\nRIDA\u00aeGENE: pi\u00f9 di un normale test per la PCR<\/title>\n<meta name=\"description\" content=\"Molecular diagnostics are an essential part of today\u2019s laboratory routine. 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