{"id":90270,"date":"2016-08-16T09:29:14","date_gmt":"2016-08-16T07:29:14","guid":{"rendered":"https:\/\/clinical.r-biopharm.com\/technologieen\/real-time-pcr\/"},"modified":"2019-07-08T15:45:18","modified_gmt":"2019-07-08T13:45:18","slug":"real-time-pcr","status":"publish","type":"page","link":"https:\/\/devclinical.r-biopharm.com\/nl\/technologieen\/real-time-pcr\/","title":{"rendered":"Real-time PCR"},"content":{"rendered":"
[mk_page_section padding_top=”30″ padding_bottom=”30″ sidebar=”sidebar-1″][vc_column width=”2\/3″][vc_column_text]Moleculaire detectiemethoden hebben de medische diagnostiek radicaal veranderd en vormen een essentieel onderdeel van de huidige laboratoriumdiagnostiek.<\/strong><\/p>\n

Op veel gebieden van infectieziekten zijn de klassieke detectiemethoden zoals kweek en immunoassays vervangen door, of aangevuld met real-time PCR (Polymerase Chain Reaction).<\/p>\n

Testprincipe van real-time PCR<\/h3>\n

Door directe detectie van DNA of RNA van het pathogeen, maakt real-time PCR het mogelijk een vroege en zeer specifieke diagnose te stellen, zodat een snelle en selectieve behandeling van de individuele pati\u00ebnt mogelijk wordt. Na DNA-isolatie worden doelgenfragmenten geamplificeerd (amplicons) en, indien aanwezig, gedetecteerd met fluorescentiehydrolyse-sondes. Het fluorescentiesignaal neemt toe met de hoeveelheid gevormde amplicons, wat gedetecteerd wordt door de optische eenheid van een real-time PCR-instrument.<\/p>\n

RIDA\u00aeGENE in \u00e9\u00e9n oogopslag<\/h3>\n

De R-Biopharm RIDA\u00aeGENE kits zijn gebaseerd op real-time PCR-technologie voor directe kwalitatieve detectie van gastro-intestinale infecties, hospital acquired infections (HAI), luchtweginfecties, seksueel overdraagbare aandoeningen (SOA) en andere parameters.[\/vc_column_text][mk_custom_list style=”mk-moon-circle-small”]\n

    \n
  • Gevoelig en specifiek: real-time PCR met TaqMan-hydrolyse-sondetechnologie<\/li>\n
  • Volledig: bevat alle noodzakelijke componenten<\/li>\n
  • Betrouwbaar: inclusief extractiecontrole die PCR-remming detecteert, reagensintegriteit bewaakt en effici\u00ebnte nucle\u00efnezuurextractie verifieert<\/li>\n
  • Flexibel: compatibel met diverse real-time PCR-instrumenten zoals de LightCycler\u00ae serie, Rotor-Gene Q, ABI7500, Mx3005P en CFX96\u2122<\/li>\n
  • Effici\u00ebnt: slechts een PCR-profiel voor zowel RNA-testen alsook DNA-testen<\/li>\n
  • Gevalideerd: CE-IVD-markering, deelname aan QCMD-, INSTAND- en UK NEQAS-kwaliteitsbeoordeling<\/li>\n<\/ul>\n[\/mk_custom_list][vc_cta h2=”Meer informatie” shape=”square” style=”flat” color=”grey” add_button=”right” btn_title=”Downloaden” btn_style=”flat” btn_shape=”square” btn_color=”inverse” btn_link=”url:https%3A%2F%2Fdevclinical.r-biopharm.com%2Fwp-content%2Fuploads%2Fsites%2F3%2F2017%2F11%2F2016-10_rida-gene_portfolio_booklet_en_lores.pdf|”]U vindt meer informatie over onze RIDA\u00aeGENE real-time PCR-kits in onze gratis brochure.[\/vc_cta][\/vc_column][vc_column width=”1\/3″][vc_column_text margin_bottom=”20″]\n

    Kies een indicatie<\/h3>\n
    <\/div>[\/vc_column_text][vc_column_text margin_bottom=”20″]\n

    Kies een diagnostisch gebied<\/h3>\n
    <\/div>[\/vc_column_text][vc_column_text margin_bottom=”40″]\n

    Kies uw real-time PCR-product<\/h3>\n
      <\/ul><\/div>[\/vc_column_text][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#f7f7f7″ padding_top=”0″ padding_bottom=”0″ sidebar=”sidebar-1″][vc_column css=”.vc_custom_1469015765963{margin-top: 25px !important;padding-bottom: 5px !important;}”][vc_column_text margin_bottom=”20″ css=”.vc_custom_1530179827972{margin-bottom: 20px !important;}”]\n

      Recent nieuws<\/h2>\n[\/vc_column_text][mk_blog_carousel cat=”233″][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#ffffff” padding_top=”0″ sidebar=”sidebar-1″][vc_column css=”.vc_custom_1467042052405{margin-top: 40px !important;}”][vc_column_text margin_bottom=”20″]\n

      U bent misschien ook ge\u00efnteresseerd in<\/h2>\n[\/vc_column_text][vc_row_inner css=”.vc_custom_1468404266697{margin-bottom: 40px !important;}”][vc_column_inner width=”1\/3″ css=”.vc_custom_1468416557833{padding-bottom: 10px !important;padding-left: 0px !important;}”][mk_flipbox_rb taxonomy=”technology” tag_technology=”qpcr-de” min_height=”200″ button_bg_color=”#4d4d4d” button_bg_hover_color=”#ffffff” button_text=”More”][\/vc_column_inner][vc_column_inner width=”1\/3″ css=”.vc_custom_1468416552613{padding-right: 7.5px !important;padding-bottom: 10px !important;padding-left: 7.5px !important;}”][mk_flipbox_rb taxonomy=”technology” tag_technology=”qpcr-de” offset=”1″ min_height=”200″ button_bg_color=”#4d4d4d” button_bg_hover_color=”#ffffff” button_text=”More”][\/vc_column_inner][vc_column_inner width=”1\/3″ css=”.vc_custom_1468416547451{padding-right: 0px !important;padding-bottom: 10px !important;}”][mk_flipbox_rb taxonomy=”technology” tag_technology=”qpcr-de” offset=”2″ min_height=”200″ button_bg_color=”#4d4d4d” button_bg_hover_color=”#ffffff” button_text=”More”][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#f7f7f7″ padding_top=”20″ padding_bottom=”20″ sidebar=”sidebar-1″][vc_column css=”.vc_custom_1468415774645{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column_text disable_pattern=”false” align=”center”]\n

      Ondersteuning voor real-time PCR<\/h2>\n

      Vragen? Maak gebruik van de expertise van ons team. Wij zijn er om u en uw organisatie tijdens het hele testproces te ondersteunen om uw succes te garanderen.[\/vc_column_text][mk_button dimension=”flat” url=”\/nl\/contact\/” align=”center” margin_bottom=”” bg_color=”#4d4d4d” btn_hover_bg=”#ffffff” btn_hover_txt_color=”#4d4d4d”]Neem contact met ons op[\/mk_button][\/vc_column][\/mk_page_section]\n<\/div>","protected":false},"excerpt":{"rendered":"

      [mk_page_section padding_top=”30″ padding_bottom=”30″ sidebar=”sidebar-1″][vc_column width=”2\/3″][vc_column_text]Moleculaire detectiemethoden hebben de medische diagnostiek radicaal veranderd en vormen een essentieel onderdeel van de huidige laboratoriumdiagnostiek. Op veel gebieden van infectieziekten zijn de klassieke detectiemethoden zoals kweek en immunoassays vervangen door, of aangevuld met real-time PCR (Polymerase Chain Reaction). Testprincipe van real-time PCR Door directe detectie van DNA of RNA […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":90040,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":"","_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","footnotes":""},"acf":[],"yoast_head":"\nReal-time PCR: moleculaire methoden voor klinische diagnostiek<\/title>\n<meta name=\"description\" content=\"Moleculaire diagnostiek is een essentieel onderdeel van de dagelijkse laboratoriumroutine. 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